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Influences of long noncoding RNA small nucleolar RNA host gene 22 on the cell proliferation, invasion and migration of oral squamous carcinoma cells by regulating microRNA-27b-3p
Zhou Jinkuo,Zhang Jinhong,Shi Xiaojing,Liu Guangshun,Jiang Lei,Liu Qianfeng
PDF(4308 KB)
PDF(4308 KB)
Influences of long noncoding RNA small nucleolar RNA host gene 22 on the cell proliferation, invasion and migration of oral squamous carcinoma cells by regulating microRNA-27b-3p
),Zhang Jinhong1,Shi Xiaojing2,Liu Guangshun2,Jiang Lei3,Liu Qianfeng1()Objective This study aimed to investigate the influences of long noncoding RNA small nucleolar RNA host gene (SNHG) 22 on the proliferation, invasion, and migration of oral squamous cell carcinoma (OSCC) cells by regulating microRNA (miR)-27b-3p. Methods Cancer tissue and paracancerous tissue specimens of 52 OSCC patients were collected. Human normal oral keratinocytes (HOK) and three kinds of human OSCC cells (CAL-27, SCC-25, and HSC-3) were cultured in vitro. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of SNHG22 and miR-27b-3p in cancer tissues, adjacent tissues, HOK cells, and three kinds of OSCC cells. SCC-25 cells were transfected and separated into the following groups: control (no transfection), si-SNHG22, si-NC, miR-27b-3p inhibitor, inhibitor-NC, si-SNHG22+inhibitor-NC, and si-SNHG22+miR-27b-3p inhibitor. The proliferation of SCC-25 cells in each group was detected by cell-counting kit 8 method, and proliferation index (PI) was detected by flow cytometry. Transwell assay was applied to detect the invasion of SCC-25 cells in each group. Scratch-area healing experiment was applied to detect the migration of SCC-25 cells in each group. Dual-luciferase experiment was applied to verify the targeting relationship between SNHG22 and miR-27b-3p. Results Compared with adjacent tissues, SNHG22 expression in OSCC cancer tissues significantly increased, and the expression of miR-27b-3p significantly decreased (P<0.05). Compared with HOK cells, SNHG22 expression significantly increased in CAL-27 cells, SCC-25 cells, and HSC-3 cells, and the expression of miR-27b-3p significantly decreased. The expression in SCC-25 cells differed the most from that in HOK cells (P<0.05). Compared with the control group, the SCC-25 cell-proliferation rate, PI, invasion number, and scratch-area healing rate in the si-SNHG22 group decreased significantly (P<0.05). The SCC-25 cell-proliferation rate, PI, invasion number, and scratch-area healing rate in the miR-27b-3p inhibitor group increased significantly (P<0.05); compared with the si-SNHG22 group. The SCC-25 cell-proliferation rate, PI, invasion number, and scratch-area healing rate in the si-SNHG22+miR-27b-3p inhibitor group increased significantly (P<0.05). Dual-luciferase experiments showed that SNHG22 had a targeting relationship with miR-27b-3p. Conclusion SNHG22 was highly expressed in OSSC, whereas miR-27b-3p was lowly expressed. SNHG22 may promote the proliferation, invasion, and migration of SCC-25 cells through sponge miR-27b-3p. The SNHG22/miR-27b-3p axis may be a new diagnostic and therapeutic target for OSCC.
long noncoding RNA small nucleolar RNA host gene 22 / microRNA-27b-3p / oral squamous cell carcinoma / proliferation / invasion / migration
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| 1 | Siegel RL, Miller KD, Jemal A. Cancer statistics, 2018[J]. CA Cancer J Clin, 2018, 68(1): 7-30. |
| 2 | Li JD, Xu XH, Zhang DD, et al. LncRNA LHFPL3-AS1 promotes oral squamous cell carcinoma growth and cisplatin resistance through targeting miR-362-5p/CHSY1 pathway[J]. Onco Targets Ther, 2021, 14: 2293-2300. |
| 3 | Tan YT, Lin JF, Li T, et al. LncRNA-mediated posttranslational modifications and reprogramming of energy metabolism in cancer[J]. Cancer Commun (Lond), 2021, 41(2): 109-120. |
| 4 | Guan N, Zheng HY, Wu XL, et al. SP1-regulated non-coding RNA SNHG22 promotes ovarian cancer growth and glycolysis[J]. Cancer Manag Res, 2021, 13: 7299-7309. |
| 5 | Zhang YX, Lu CL, Cui HW. Long non-coding RNA SNHG22 facilitates hepatocellular carcinoma tumori-genesis and angiogenesis via DNA methylation of microRNA miR-16-5p[J]. Bioengineered, 2021, 12(1): 7446-7458. |
| 6 | Venkatesh J, Wasson MD, Brown JM, et al. Lnc-RNA-miRNA axes in breast cancer: novel points of interaction for strategic attack[J]. Cancer Lett, 2021, 509: 81-88. |
| 7 | Xiao Z, Li JY, Jin QS, et al. Long non-coding RNA OIP5-AS1 contributes to cisplatin resistance of oral squamous cell carcinoma through the miR-27b-3p/TRIM14 axis[J]. Exp Ther Med, 2021, 21(4): 408. |
| 8 | Bhan A, Soleimani M, Mandal SS. Long noncoding RNA and cancer: a new paradigm[J]. Cancer Res, 2017, 77(15): 3965-3981. |
| 9 | Cui XF, Zhang HY, Chen T, et al. Long noncoding RNA SNHG22 induces cell migration, invasion, and angiogenesis of gastric cancer cells via microRNA-361-3p/HMGA1/Wnt/β?catenin axis[J]. Cancer Ma-nag Res, 2020, 12: 12867-12883. |
| 10 | Li ZW, Zhang TY, Yue GJ, et al. Small nucleolar RNA host gene 22 (SNHG22) promotes the progression of esophageal squamous cell carcinoma by miR-429/SESN3 axis[J]. Ann Transl Med, 2020, 8(16): 1007. |
| 11 | Shao TT, Wang GJ, Chen H, et al. Survey of mi-RNA-miRNA cooperative regulation principles across cancer types[J]. Brief Bioinform, 2019, 20(5): 1621-1638. |
| 12 | Zhou RS, Zhang EX, Sun QF, et al. Integrated analysis of lncRNA-miRNA-mRNA ceRNA network in squamous cell carcinoma of tongue[J]. BMC Cancer, 2019, 19(1): 779. |
| 13 | Mao XQ, Ji T, Liu AG, et al. ELK4-mediated lnc-RNA SNHG22 promotes gastric cancer progression through interacting with EZH2 and regulating miR-200c-3p/Notch1 axis[J]. Cell Death Dis, 2021, 12(11): 957. |
| 14 | Yao JN, Wang CF, Dong XY, et al. lncRNA SNHG22 sponges miR?128?3p to promote the progression of colorectal cancer by upregulating E2F3[J]. Int J Oncol, 2021, 59(3): 71. |
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