脱卤杆菌介导的厌氧微生物富集菌群对1,2,4-三氯苯的降解特性

吕良华; 乔文静; 张晗; 叶淑君; 吴吉春; 王水; 蒋建东

doi:10.13745/j.esf.sf.2022.10.41

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地学前缘 ›› 2024, Vol. 31 ›› Issue (2) : 472-480. DOI: 10.13745/j.esf.sf.2022.10.41

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脱卤杆菌介导的厌氧微生物富集菌群对1,2,4-三氯苯的降解特性

吕良华 ¹^,²^,³ ,
乔文静 ³^,^* ,
张晗 ³ ,
叶淑君 ⁴ ,
吴吉春 ⁴ ,
王水 ¹^,² ,
蒋建东 ³

作者信息 +

Degradation of 1,2,4-trichlorobenzene by an anaerobic enrichment culture mediated by Dehalobacter species

Lianghua LÜ ¹^,²^,³ ,
Wenjing QIAO ³^,^* ,
Han ZHANG ³ ,
Shujun YE ⁴ ,
Jichun WU ⁴ ,
Shui WANG ¹^,² ,
Jiandong JIANG ³

Author information +

History +

摘要

1,2,4-三氯苯是我国工业污染场地土壤和地下水中典型的有机污染物,具有持久性、生物蓄积性和高毒性特点,对生态环境和人体健康危害巨大。1,2,4-三氯苯密度比水大,容易迁移至深部厌氧区域,因此,开展1,2,4-三氯苯厌氧微生物降解与修复研究具有重要实际应用价值。本文通过长期富集培养,获得一份可以稳定地将1,2,4-三氯苯还原脱氯至1,4-二氯苯,再进一步还原脱氯至氯苯的厌氧菌液。通过16S rRNA基因扩增子测序及引物特异性的定量PCR实验,证明厚壁菌门的脱卤杆菌属细菌(Dehalobacter species)是1,2,4-三氯苯和1,4-二氯苯厌氧还原脱卤的功能菌株,其生长率为(1.68±0.8)×10⁶ copies·μmol^-1(释放的氯离子)。通过PCR扩增,获得Dehalobacter菌株的16S rRNA基因序列,构建了系统发育树。本研究可为1,2,4-三氯苯污染场地开展原位厌氧微生物修复提供菌株资源和理论指导。

Abstract

1,2,4-Trichlorobenzene (TCB) is a typical organic contaminant in soil and groundwater at industrial plant in China. 1,2,4-TCB is recalcitrant, bio-accumulative and highly toxic, posing serious threats to the environment and human health. 1,2,4-TCB is denser than water and easily penetrates into an anaerobic environment. Therefore, understanding the anaerobic biotransformation of 1,2,4-TCB is of great practical significance to on-site remediation. In this study, an anaerobic enrichment culture, which can sustainably dechlorinate 1,2,4-TCB to MCB via 1,4-DCB, is obtained. 16S rRNA gene amplicon sequencing and qPCR demonstrated Dehalobacter is responsible for the observed reductive dechlorination with a growth yield of (1.68±0.8)×10⁶ copies per μmol released Cl^-. A complete 16S rRNA gene sequence was obtained through PCR and a corresponding phylogenetic tree was constructed. This study provides a reference for the enrichment culture technique as well as theoretical guidance for the in-situ anaerobic bioremediation of contaminated sites.

关键词

1,2,4-三氯苯 / 地下水污染 / 生物修复 / 脱卤杆菌属 / 还原脱卤

Key words

1,2,4-trichlorobenzene / groundwater contamination / bioremediation / Dehalobacter / reductive dechlorination

中图分类号

X523;X172

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导出引用

吕良华 , 乔文静 , 张晗 , 等. 脱卤杆菌介导的厌氧微生物富集菌群对1,2,4-三氯苯的降解特性. 地学前缘. 2024, 31(2): 472-480 https://doi.org/10.13745/j.esf.sf.2022.10.41

Lianghua LÜ, Wenjing QIAO, Han ZHANG, et al. Degradation of 1,2,4-trichlorobenzene by an anaerobic enrichment culture mediated by Dehalobacter species[J]. Earth Science Frontiers. 2024, 31(2): 472-480 https://doi.org/10.13745/j.esf.sf.2022.10.41

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脚注

基金

国家重点研发计划项目(2018YFC1802501)

国家自然科学基金项目(42007214)

国家自然科学基金项目(42077174)

中国博士后科学基金项目(2021M691614)

江苏省“双创博士”(JSSCBS20210278)

江苏省“333高层次人才培养工程”科研项目(680803125)

江苏省卓越博士后计划项目(2023ZB141)

江苏省地下水环境状况调查项目

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Received	Revised	Published
2022-10-13	2022-11-10	2024-03-25
Issue Date
2025-03-24

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