目的 构建“知识-案例-思政”三位一体的中医药院校细胞工程课堂教学模式。 方法 以浙江中医药大学2020级医学实验技术专业学生为观察组，以2019级医学实验技术专业学生为对照组，分别采用案例教学和传统授课模式开展教学。以学生成绩和学生能力素养提升的问卷结果对教学效果进行评价。 结果 “知识-案例-思政”三位一体的细胞工程课堂教学模式可提高学生的期末成绩和总评成绩平均分，特别是期末成绩的优秀率和良好率显著提高；新教学模式可激发学生的学习兴趣、自我展示和科研创新意识，培养学生的集体责任心和荣誉感；有利于学生课程学习、专业实践、团队合作、沟通交流、分析解决问题等能力的提升。 结论 以案例为载体的教学可促进学生对知识的掌握，并提升学生的综合能力和素养。

Objective To construct a “knowledge-case-ideology and politics” trinity classroom teaching model for the Cell Engineering course under the background of traditional Chinese medicine （TCM）. Methods Students majoring in medical experimental technology from the 2020 cohort were selected as the observation group， and students from the 2019 cohort were used as the control group. The observation group adopted the case-based teaching method， while the control group used the traditional lecture-based method. The teaching effects were evaluated through student performance in exams and the results of a questionnaire assessing the improvement of students' abilities and qualities. Results The “knowledge-case-ideology and politics” teaching model significantly improved both final grades and overall scores， particularly enhancing the proportion of students achieving excellent and good results. This new teaching model stimulated students' interest in learning， encouraged self-presentation， fostered innovation in scientific research， and developed a sense of collective responsibility and honor. It also contributed to improving students' abilities in course learning， professional practice， teamwork， communication， and problem-solving. Conclusion Case-based teaching effectively promotes students' understanding of knowledge while enhancing their comprehensive abilities and personal development.