玉米GLP家族基因鉴定及其响应丛枝菌根共生表达

春建惠, 董文龙, 屠元超, 刘芳, 徐云剑

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中国高校科技期刊信息汇聚平台
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植物研究 ›› 2025, Vol. 45 ›› Issue (03) : 406-418. DOI: 10.7525/j.issn.1673-5102.2025.03.011
研究论文

玉米GLP家族基因鉴定及其响应丛枝菌根共生表达

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Identification of the Maize GLP Family Genes and Their Expression in Response to Arbuscular Mycorrhizal Symbiosis

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摘要

类萌发素蛋白（germin-like proteins，GLPs）是一类高度保守的胁迫响应蛋白，可特异性响应菌根共生。该研究基于玉米（Zea mays）B73全基因组信息，利用生物信息学方法对ZmGLP基因家族成员进行鉴定及特征分析，利用转录组数据分析ZmGLP基因在丛枝菌根真菌（AMF）共生下的表达模式。该研究鉴定了45个ZmGLP基因，它们分布于9条染色体上，存在25个串联复制基因；系统发育树将ZmGLP基因分为5个亚家族；不同ZmGLP基因组织表达模式存在较大差异。启动子顺式作用元件分析显示，ZmGLP基因启动子包含响应光、胁迫和生长发育相关的元件，ZmGLP4-8启动子含有菌根响应元件MYCS，20个ZmGLP基因启动子包含潜在的菌根响应元件GCCGGC；基于接种AMF后不同时间的玉米根部转录组数据，发现12个ZmGLP基因的表达在接种AMF后出现了显著变化；其中ZmGLP3-3、ZmGLP4-8、ZmGLP4-16、ZmGLP4-20、ZmGLP5-1、ZmGLP6-1在共生后期显著上调表达，且与已报道的共生相关GLP基因在不同的进化分支，暗示这些基因可能参与菌根共生后期相关的功能。ZmGLP3-3功能研究显示，相较于野生型玉米植株，突变体zmglp3-3菌根定殖率显著降低。综上，该研究为共生相关ZmGLP基因挖掘提供了理论基础。

Abstract

Germin-like proteins （GLPs） are a class of highly conserved stress-responsive proteins that can specifically respond to symbiotic interactions. This study， based on the whole-genome information of maize B73， used bioinformatics approaches to identify and analyze the characteristics of the ZmGLP gene family. The expression patterns of ZmGLP genes during arbuscular mycorrhizal fungi（AMF） symbiosis were examined by using transcriptome data. This study identified a total of 45 ZmGLP genes， which were distributed across nine chromosomes， with 25 of the genes being tandem duplications. By analysis of a phylogenetic tree， ZmGLP genes were classified into five distinct subfamilies. Notably， there were significant differences in tissue expression patterns among different ZmGLP genes. Promoter cis-element analysis showed that ZmGLP promoters contained elements responsive to light， stress， and growth and development. Interestingly， the promoter of ZmGLP4-8 contained the mycorrhiza-responsive element MYCS， and 20 ZmGLP promoters contained the potential mycorrhiza-responsive element GCCGGC. Analysis of transcriptome data from maize roots at different days after inoculation with AMF revealed that the expression of 12 ZmGLP genes was significantly changed following inoculation with AMF. Among them， ZmGLP3-3， ZmGLP4-8， ZmGLP4-16， ZmGLP4-20， ZmGLP5-1， and ZmGLP6-1 were significantly upregulated in expression during the late stage of symbiosis. These genes were located in different evolutionary branches compared to the reported symbiosis-related GLP genes， suggesting that these genes may be involved in functions related to the late stage of mycorrhizal symbiosis. Functional studies of ZmGLP3-3 showed that， compared to wild-type maize plants， the mycorrhizal symbiosis rate of the mutant zmglp3-3 was significantly reduced. In summary， this study provided a comprehensive theoretical basis for the exploration of symbiosis-related ZmGLP genes in maize， offering insights into their potential roles and functions in plant-fungal interactions.

关键词

丛枝菌根真菌 / 类萌发素蛋白 / 基因家族 / 菌根共生 / 表达分析

Key words

arbuscular mycorrhizal fungi / germin-like proteins / gene family / mycorrhizal symbiosis / expression analysis

中图分类号

Q939

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春建惠 , 董文龙 , 屠元超 , 等. 玉米GLP家族基因鉴定及其响应丛枝菌根共生表达. 植物研究. 2025, 45(03): 406-418 https://doi.org/10.7525/j.issn.1673-5102.2025.03.011
CHUN Jianhui, DONG Wenlong, TU Yuanchao, et al. Identification of the Maize GLP Family Genes and Their Expression in Response to Arbuscular Mycorrhizal Symbiosis[J]. Bulletin of Botanical Research. 2025, 45(03): 406-418 https://doi.org/10.7525/j.issn.1673-5102.2025.03.011

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基金

云南省科技计划项目(202401BF070001-002)
云南省科技计划项目(202301AT070106)
云南省教育厅科学研究基金项目(KC-24248854)

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