古朴树实生苗生长和生理对土著丛枝菌根真菌接种的响应

刘武, 阳雅荧, 龚宁, 邹紫薇, 王艺, 陈保冬, 王琼, 刘玮

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植物研究 ›› 2025, Vol. 45 ›› Issue (03) : 393-405. DOI: 10.7525/j.issn.1673-5102.2025.03.010
研究论文

古朴树实生苗生长和生理对土著丛枝菌根真菌接种的响应

作者信息 +

Growth and Physiological Responses of Ancient Celtis sinensis Seedlings to the Inoculation of Indigenous Arbuscular Mycorrhizal Fungi

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摘要

城市中的古树受自身和环境因素影响,生长受限,接种古树根际土壤中的土著优势丛枝菌根(AM)真菌或可成为一种新的古树复壮方法。以上海市古朴树(Celtis sinensis)根际土筛选、扩繁的2种土著AM真菌菌剂(层状近明球囊霉(Claroideoglomus lamellosum)和摩西斗管囊霉(Funneliformis mosseae))为试验材料,接种至古朴树实生苗盆栽中进行验证。结果表明:接种后实生苗的株高、茎粗和叶面积较对照均显著增加,叶片数量也显著增多;混合接种显著增加了实生苗全株生物量、根尖数量及器官中N、P养分积累量,并能提高实生苗的光合能力,促进叶绿素生成;接种2种菌种均能提升超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,增强实生苗抗性。基于隶属模糊函数综合法得出的评价结果可知,朴树实生苗促生效果最好的接种方式为混合接种。该研究结果可为古朴树复壮菌剂的应用提供一定参考。

Abstract

Ancient trees in urban areas are affected by their own and environmental factors, resulting in growth restriction. Inoculation with indigenous dominant arbuscular mycorrhizal(AM) fungi in the rhizosphere soil of ancient trees may be a new method of ancient tree rejuvenation. Two indigenous AM fungal mycorrhizal agents(Claroideoglomus lamellosum and Funneliformis mosseae) were screened and propogated from the rhizosphere soil of Celtis sinensis trees in Shanghai, and were verified by inoculation into pots of C. sinensis seedlings. The results showed that plant height, stem diameter and leaf area of the inoculated seedlings increased significantly compared to the control, and the number of leaves also increased significantly. The mixed inoculation had a significant effect on the biomass of the whole plant and number of root tips. The accumulation of nitrogen(N) and phosphorus(P) nutrients, photosynthetic capacity and chlorophyll content of the seedlings were improved as compared with that of the control, respectively. Inoculation with both strains enhanced the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in leaves and enhanced the resistance of live seedlings. Based on a comprehensive evaluation of the fuzzy membership function, an optimal inoculation strategy for promoting the growth of ancient C. sinensis seedlings was identified as FM+CL. This work provided a valuable reference for the application of indigenous AM fungi agents in the rejuvenation of ancient trees.

关键词

菌根共生 / 古树复壮 / 促生效应 / 隶属模糊函数评价

Key words

mycorrhizal symbiosis / ancient tree rejuvenation / growth-promoting effects / fuzzy membership function evaluation

中图分类号

Q938.1

引用本文

导出引用
刘武 , 阳雅荧 , 龚宁 , . 古朴树实生苗生长和生理对土著丛枝菌根真菌接种的响应. 植物研究. 2025, 45(03): 393-405 https://doi.org/10.7525/j.issn.1673-5102.2025.03.010
LIU Wu, YANG Yaying, GONG Ning, et al. Growth and Physiological Responses of Ancient Celtis sinensis Seedlings to the Inoculation of Indigenous Arbuscular Mycorrhizal Fungi[J]. Bulletin of Botanical Research. 2025, 45(03): 393-405 https://doi.org/10.7525/j.issn.1673-5102.2025.03.010

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基金

上海市绿化和市容管理局科技攻关项目(G230505)
江西省“双千计划”项目(jxsq2018102090)

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