丛枝菌根真菌培养与应用研究进展

汪伯晏, 陈金, 程齐修, 包月明, 王海宁, 秦锐, 李晓玉

PDF(2040 KB)
PDF(2040 KB)
植物研究 ›› 2025, Vol. 45 ›› Issue (03) : 361-370. DOI: 10.7525/j.issn.1673-5102.2025.03.007
综述文章

丛枝菌根真菌培养与应用研究进展

作者信息 +

Progress of Research on Culture and Application of Arbuscular Mycorrhizal Fungi

Author information +
History +

摘要

丛枝菌根真菌(AMF)是土壤生态系统中关键的微生物群落之一,研究其繁殖技术及应用对于提升农业生产效率与实现农业可持续性具有深远意义。该文综述了AMF的共生机制及其在扩繁体系和菌剂应用方面的最新研究进展。探讨了AMF与植物根系建立共生关系的分子机制;分析了AMF扩繁体系的优化策略,包括无菌培养技术、基质选择和环境控制等关键因素;讨论了AMF菌剂在实际生产中的应用潜力,包括提高作物产量、增强植物抗逆性和改善土壤结构等,并阐述了当前AMF应用领域存在的实际问题与今后的研究方向。该项研究旨在为进一步理解丛枝菌根真菌的重要性提供参考,并为未来新型微生物菌剂的开发及其在农业生态系统中的应用奠定基础。

Abstract

Arbuscular mycorrhizal fungi(AMF) are one of the crucial microbial communities in the soil ecosystem. Researches on AMF reproductive techniques and their applications in practice have profound significance for elevating agricultural production efficiency and sustainability. This paper reviewed the latest research advancements in the symbiotic mechanism of AMF and its application in propagation systems and microbial inoculants. It explored the molecular mechanism through which AMF establish symbiotic relationships with plant roots; analyzed the optimization strategies for AMF propagation systems, encompassing key factors such as aseptic culture techniques, substrate selection, and environmental control; discussed the application potential of AMF inoculants in actual production, including enhancing crop yields, strengthening plant stress resistance, and improving soil structure. It highlighted the practical issues existing in the current application domains of AMF and the future research directions. The purpose was to offer a reference for further comprehension of the significance of arbuscular mycorrhizal fungi and lay the foundation for the development of novel microbial inoculants and their application in agricultural ecosystems in the future.

关键词

丛枝菌根真菌 / 共生机制 / 扩繁体系 / 菌剂应用

Key words

arbuscular mycorrhizal fungi / symbiotic mechanism / propagation system / application of microbial inoculants

中图分类号

Q948.12 / S154.3

引用本文

导出引用
汪伯晏 , 陈金 , 程齐修 , . 丛枝菌根真菌培养与应用研究进展. 植物研究. 2025, 45(03): 361-370 https://doi.org/10.7525/j.issn.1673-5102.2025.03.007
WANG Boyan, CHEN Jin, CHENG Qixiu, et al. Progress of Research on Culture and Application of Arbuscular Mycorrhizal Fungi[J]. Bulletin of Botanical Research. 2025, 45(03): 361-370 https://doi.org/10.7525/j.issn.1673-5102.2025.03.007

参考文献

1
REDECKER D KODNER R GRAHAM L E.Glomalean fungi from the Ordovician[J].Science2000289(5486):1920-1921.
2
SMITH S E READ D.Mycorrhizal symbiosis[M].3rd ed.New York:Academic Press,2008.
3
SPORTES A HÉRICHÉ M BOUSSAGEON R,et al.A historical perspective on mycorrhizal mutualism emphasizing arbuscular mycorrhizas and their emerging challenges[J].Mycorrhiza202131:637-653.
4
YU L M ZHANG Z F ZHOU L W.Advances in the studies on symbiotic arbuscular mycorrhizal fungi of traditional Chinese medicinal plants[J].Biocell202246(12):2559-2573.
5
BRUNDRETT M C TEDERSOO L.Evolutionary history of mycorrhizal symbioses and global host plant diversity[J].New Phytologist2018220(4):1108-1115.
6
SCHAARSCHMIDT S HAUSE B.Apoplastic invertases:multi-faced players in the arbuscular mycorrhization[J].Plant Signaling & Behavior20083(5):317-319.
7
SABOOR A ALI M A HUSSAIN S,et al.Zinc nutrition and arbuscular mycorrhizal symbiosis effects on maize (Zea mays L.) growth and productivity[J].Saudi Journal of Biological Sciences202128(11):6339-6351.
8
ZHANG Y C ZOU Y N LIU L P,et al.Common mycorrhizal networks activate salicylic acid defense responses of trifoliate orange (Poncirus trifoliata)[J].Journal of Integrative Plant Biology201961(10):1099-1111.
9
YU H LIU X Y YANG C,et al.Co-symbiosis of arbuscular mycorrhizal fungi (AMF) and diazotrophs promote biological nitrogen fixation in mangrove ecosystems[J].Soil Biology and Biochemistry2021161:108382.
10
LI X R SHI F ZHOU M,et al.Migration and accumulation of microplastics in soil-plant systems mediated by symbiotic microorganisms and their ecological effects[J].Environment International2024191:108965.
11
GUL S, WHALEN J K.Biochemical cycling of nitrogen and phosphorus in biochar-amended soils[J].Soil Biology and Biochemistry2016103:1-15.
12
ZHOU J C ZHANG L FENG G,et al.Arbuscular mycorrhizal fungi have a greater role than root hairs of maize for priming the rhizosphere microbial community and enhancing rhizosphere organic P mineralization[J].Soil Biology and Biochemistry2022171:108713.
13
BESSERER A PUECH-PAGÈS V KIEFER P,et al.Strigolactones stimulate arbuscular mycorrhizal fungi by activating mitochondria[J].PLoS Biology20064(7):e226.
14
JIANG F ZHANG L ZHOU J,et al.Arbuscular mycorrhizal fungi enhance mineralisation of organic phosphorus by carrying bacteria along their extraradical hyphae[J].New Phytologist2021230(1):304-315.
15
RICH M K NOURI E COURTY P E,et al.Diet of arbuscular mycorrhizal fungi:bread and butter?[J].Trends in Plant Science201722(8):652-660.
16
HUANG H LIU S J DU Y,et al.Carbon allocation mediated by arbuscular mycorrhizal fungi alters the soil microbial community under various phosphorus levels[J].Fungal Ecology202362:101227.
17
李少杰,肖清山,宋福强,等.丛枝菌根(AM)真菌扩培技术研究进展[J].中国农学通报202238(9):115-122.
LI S J XIAO Q S SONG F Q,et al.Propagation of arbuscular mycorrhizal fungi:a review[J].Chinese Agricultural Science Bulletin202238(9):115-122.
18
HAY M J M.A technique for growing mycorrhizal clover in solution culture[J].New Zealand Journal of Agricultural Research198124(3/4):371-372.
19
ELMES R P MOSSE B.Vesicular-arbuscular endomycorrhizal inoculum production.II.Experiments with maize (Zea mays) and other hosts in nutrient flow culture[J].Canadian Journal of Botany198462(7):1531-1536.
20
黄玉丹,张淑彬,李琳,等.丛枝菌根真菌繁殖体的高效扩繁[J].微生物学通报202350(2):503-513.
HUANG Y D ZHANG S B LIN L,et al.Efficient propagation of arbuscular mycorrhizal fungal propagules[J].Microbiology China202350(2):503-513.
21
KLIRONOMOS J N.Variation in plant response to native and exotic arbuscular mycorrhizal fungi[J].Ecology200384(9):2292-2301.
22
马俊卿,侯宁,孙晨瑜,等.宿主不同对丛枝菌根真菌扩繁效应的影响[J].中国农学通报202238(1):7-14.
MA J Q HOU N SUN C Y,et al.Effects of different hosts on propagation of arbuscular mycorrhizal fungi[J].Chinese Agricultural Science Bulletin202238(1):7-14.
23
李媛媛,王晓娟,豆存艳,等.四种宿主植物及其不同栽培密度对AM真菌扩繁的影响[J].草业学报201322(5):128-135.
LI Y Y WANG X J DOU C Y,et al.Effects of four host plants and different cultivation densities on the propagation of arbuscular mycorrhizal fungi[J].Acta Prataculturae Sinica201322(5):128-135.
24
冉海燕,江龙.AM真菌离体双重培养研究进展[J].山地农业生物学报201635(2):62-67.
RAN H Y JIANG L.The research progress of AM fungi in vitro dual culture[J].Journal of Mountain Agriculture and Biology201635(2):62-67.
25
MOSSE B.The establishment of vesicular-arbuscular mycorrhiza under aseptic conditions[J].Journal of General Microbiology196227(3):509-520.
26
王晶晶,孙淑斌,徐国华.丛枝菌根真菌侵染番茄离体毛状根双重培养体系的建立[J].菌物学报201029(1):68-74.
WANG J J SUN S B XU G H.An in vitro dual culturing system established with tomato hairy roots and arbuscular mycorrhizal fungi[J].Mycosystema201029(1):68-74.
27
SRINIVASAN M KUMAR K KUMUTHA K,et al.Establishing monoxenic culture of arbuscular mycorrhizal fungus Glomus intraradices through root organ culture[J].Journal of Applied and Natural Science20146(1):290-293.
28
HILDEBRANDT U JANETTA K BOTHE H.Towards growth of arbuscular mycorrhizal fungi independent of a plant host[J].Applied and Environmental Microbiology200268(4):1919-1924.
29
KAMEOKA H TSUTSUI I SAITO K,et al.Stimulation of asymbiotic sporulation in arbuscular mycorrhizal fungi by fatty acids[J].Nature Microbiology20194(10):1654-1660.
30
SUGIURA Y AKIYAMA R TANAKA S,et al.Myristate can be used as a carbon and energy source for the asymbiotic growth of arbuscular mycorrhizal fungi[J].Proceedings of the National Academy of Sciences of the United States of America2020117(41):25779-25788.
31
TANAKA S HASHIMOTO K KOBAYASHI Y,et al.Asymbiotic mass production of the arbuscular mycorrhizal fungus Rhizophagus clarus [J].Communications Biology20225(1):43.
32
WU Q S LIU C Y ZHANG D J,et al.Mycorrhiza alters the profile of root hairs in trifoliate orange[J].Mycorrhiza201626(3):237-247.
33
MARLEAU J DALPÉ Y ST-ARNAUD M,et al.Spore development and nuclear inheritance in arbuscular mycorrhizal fungi[J].BMC Evolutionary Biology201111:51.
34
SHI X N COLLADO C E HERNÁNDEZ R.Improve Cannabis sativa micropropagation through increasing air change rate in photoautotrophic and traditional tissue culture[J].Scientia Horticulturae2024333:113238.
35
LIU W K YANG Q C.Integration of mycorrhization and photoautotrophic micropropagation in vitro:feasibility analysis for mass production of mycorrhizal transplants and inoculants of arbuscular mycorrhizal fungi[J].Plant Cell,Tissue and Organ Culture200895:131-139.
36
PARÉ L BANCHINI C HAMEL C,et al.A simple and low-cost technique to initiate single-spore cultures of arbuscular mycorrhizal fungi using a superabsorbent polymer[J].Symbiosis202288(1):61-73.
37
AFFOKPON A COYNE D L LAWOUIN L,et al.Effectiveness of native West African arbuscular mycorrhizal fungi in protecting vegetable crops against root-knot nematodes[J].Biology and Fertility of Soils201147:207-217.
38
BAUM C EL-TOHAMY W GRUDA N.Increasing the productivity and product quality of vegetable crops using arbuscular mycorrhizal fungi:a review[J].Scientia Horticulturae2015187:131-141.
39
LI Y D NAN Z B MATTHEW C,et al.Arbuscular mycorrhizal fungus changes alfalfa (Medicago sativa) metabolites in response to leaf spot (Phoma medicaginis) infection,with subsequent effects on pea aphid (Acyrthosiphon pisum) behavior[J].New Phytologist2023239(1):286-300.
40
PASKOVIĆ I SOLDO B BAN S G,et al.Fruit quality and volatile compound composition of processing tomato as affected by fertilisation practices and arbuscular mycorrhizal fungi application[J].Food Chemistry2021359:129961.
41
CHENG X F XIE M M LI Y,et al.Effects of field inoculation with arbuscular mycorrhizal fungi and endophytic fungi on fruit quality and soil properties of Newhall navel orange[J].Applied Soil Ecology2022170:104308.
42
HARTMANN M,SIX J.Soil structure and microbiome functions in agroecosystems[J].Nature Reviews Earth & Environment20234(1):4-18.
43
WU Q S WANG S SRIVASTAVA A K.Mycorrhizal hyphal disruption induces changes in plant growth,glomalin-related soil protein and soil aggregation of trifoliate orange in a core system[J].Soil and Tillage Research2016160:82-91.
44
WANG B Y XIAO Q C GENG X W,et al.Arbuscular mycorrhizal fungi alter rhizosphere bacterial diversity,network stability and function of lettuce in barren soil[J].Scientia Horticulturae2024323:112533.
45
ZHANG Z F MALLIK A ZHANG J C,et al.Effects of arbuscular mycorrhizal fungi on inoculated seedling growth and rhizosphere soil aggregates[J].Soil and Tillage Research2019194:104340.
46
SOLÍS-RAMOS L Y COTO-LÓPEZ C ANDRADE-TORRES A.Role of arbuscular mycorrhizal symbiosis in remediation of anthropogenic soil pollution[J].Symbiosis202184(3):321-336.
47
BI Y L DU X P TIAN L X,et al.Effects of an arbuscular mycorrhizal fungus on Amorpha fruticosa roots and soil preferential flow in an arid area of opencast coal mine waste[J].Soil and Tillage Research2025245:106321.
48
GIRI B SAXENA B.Response of arbuscular mycorrhizal fungi to global climate change and their role in terrestrial ecosystem C and N cycling[M]//VARMA A,PRASAD R,TUTEJA N.Mycorrhiza-function,diversity,state of the art.Cham:Springer,2017:305-327.
49
BOYNO G YERLI C ÇAKMAKCI T,et al.The effect of arbuscular mycorrhizal fungi on carbon dioxide (CO2) emission from turfgrass soil under different irrigation intervals[J].Journal of Water and Climate Change202415(2):541-553.
50
TANG B MAN J LEHMANN A,et al.Arbuscular mycorrhizal fungi benefit plants in response to major global change factors[J].Ecology Letters202326(12):2087-2097.
51
MASON A R G LOWE A J BRIEN C,et al.Response of wheat to arbuscular mycorrhizal fungi inoculation and biochar application:implications for soil carbon sequestration[J].Soil Biology and Biochemistry2024199:109611.
52
LI Z S LI Y Y ZHANG Q,et al.Synergistic mechanisms of AMF and biochar driving rhizosphere fungal community in shallot in barren soil[J].Horticultural Plant Journal202410(5):1252-1256.
53
JIN X H LIU K ZHANG N L,et al.The combined application of arbuscular mycorrhizal fungi and biochar improves the Cd tolerance of Cinnamomum camphora seedlings[J].Rhizosphere202431:100939.
54
龙勇,林叶春,蔡凯,等.添加菜籽饼或菜籽油对植烟土壤差异代谢物的影响[J].湖南农业科学2024(9):63-67.
LONG Y LIN Y C CAI K,et al.Effects of rapeseed meal or oil on differential metabolites in tobacco-planting soil[J].Hunan Agricultural Sciences2024(9):63-67.
55
SRIVASTAVA A K SINGH S MARATHE R A.Organic citrus:soil fertility and plant nutrition[J].Journal of Sustainable Agriculture200219(3):5-29.
56
QIU L GAO W L WANG Z G,et al.Citric acid and AMF inoculation combination-assisted phytoextraction of vanadium (V) by Medicago sativa in V mining contaminated soil[J].Environmental Science and Pollution Research202128:67472-67486.
57
KHAFAGI E Y EL-ABEID S E SOLIMAN M S,et al.Role of arbuscular mycorrhizae fungi and humic acid in controlling root and crown rot of strawberry[J].Plant Pathology Journal201817(2):65-74.
58
ZHANG J L ZHAO R T LI X,et al.Potential of arbuscular mycorrhizal fungi for soil health:a review[J].Pedosphere202434(2):279-288.
59
El-SHARKAWY H H A ABBAS M S SOLIMAN A S,et al.Synergistic effect of growth-promoting microorganisms on bio-control of Fusarium oxysporum f.sp.pisi,growth,yield,physiological and anatomical characteristics of pea plants[J].Pesticide Biochemistry and Physiology2021178:104939.
60
ZHANG Q CHEN J LI Y Y,et al.Effects of interactions between arbuscular mycorrhizal fungi and bacteria on the growth of Lotus corniculatus L.:from the perspective of regulating rhizosphere fungal community[J].Pedosphere202434(2):411-423.
61
DE CARVALHO NETA S J ARAÚJO V L V P FRACETTO F J C,et al.Growth-promoting bacteria and arbuscular mycorrhizal fungus enhance maize tolerance to saline stress[J].Microbiological Research2024284:127708.
62
NAFADY N A SULTAN R EL-ZAWAHRY A M,et al.Effective and promising strategy in management of tomato root-knot nematodes by Trichoderma harzianum and arbuscular mycorrhizae[J].Agronomy202212(2):315.
63
YUAN S F LI M Y FANG Z Y,et al.Biological control of tobacco bacterial wilt using Trichoderma harzianum amended bioorganic fertilizer and the arbuscular mycorrhizal fungi Glomus mosseae [J].Biological Control201692:164-171.
64
QIN Z F ZHANG H Y FENG G,et al.Soil phosphorus availability modifies the relationship between AM fungal diversity and mycorrhizal benefits to maize in an agricultural soil[J].Soil Biology and Biochemistry2020144:107790.
65
FENG Z W LIU X D FENG G D,et al.Linking lipid transfer with reduced arbuscule formation in tomato roots colonized by arbuscular mycorrhizal fungus under low pH stress[J].Environmental Microbiology202022(3):1036-1051.
66
GAI J P FAN J Q ZHANG S B,et al.Direct effects of soil cadmium on the growth and activity of arbuscular mycorrhizal fungi[J].Rhizosphere20187:43-48.

基金

国家重点研发计划项目(2023YFD1901002)

评论

PDF(2040 KB)

Accesses

Citation

Detail

段落导航
相关文章

/