PDF(5152 KB)
转PsnNAC007高耐旱性小黑杨种质创制及其特性分析
武晓倩, 何旭, 高境烩, 李爽
PDF(5152 KB)
PDF(5152 KB)
转PsnNAC007高耐旱性小黑杨种质创制及其特性分析
)Germplasm Innovation and Characteristic Analysis of Transgenic PsnNAC007Populus simonii×P. nigra with High Drought Tolerance
)小黑杨（Populus simonii×P. nigra）是东北地区速生、耐寒、材性优良的树种。为了创制适种范围广、耐旱性状明显改良的林木新种质，利用小黑杨为材料，以干旱胁迫关键响应因子PsnNAC007转录因子为对象，创制了小黑杨过表达OE-PsnNAC007转基因植株。对OE-PsnNAC007转基因植株的生长指标、干旱胁迫适应能力、净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）、导水率指标、细胞形态和木材组分进行分析。结果显示：与野生型小黑杨相比，转基因植株的生长情况无明显差异，而干旱胁迫成活率提高了26.15%。在干旱胁迫条件下，转基因植株气孔导度减小、蒸腾速率下降、水分利用效率明显提高，植株茎干的导水率损失显著减少。解剖学分析发现，PsnNAC007的过量表达导致植株茎干产生更多、更小的导管细胞，这种细胞特性有利于植株在干旱条件下维持水分连续高效的运输。木材组分分析发现，转基因植株茎干木质素沉积明显增强，构成纤维素、半纤维素的单糖含量均无明显变化。
Populus simonii×P. nigra is a fast-growing and cold-tolerant tree species with elite wood properties in northeast China. In order to produce a new forest germplasm with more widely planting and improved drought tolerance， this study successfully generated transgenic P. simonii×P. nigra using PsnNAC007， a key drought response transcription factor gene. Growth characteristics， drought tolerant ability， net photosynthetic rate（Pn）， transpiration rate（Tr）， stomatal conductance（Gs）， water conductance， cell morphology and wood components of overexpressed PsnNAC007 transgenic plants were analyzed respectively. The results showed that there was no significant difference in growth between transgenic and wild-type plants， but drought survival rate was increased by 26.15%. Under drought condition， transgenic plants exhibited reduced Cleaf and Tr， improved water use efficiency， and lower water conductance loss. Anatomical analysis showed that the overexpression of PsnNAC007 led to more and smaller stem xylem vessels， which were conducive to the continuous and efficient water transport in plants under drought conditions. Based on wood components analysis， lignin deposition in stems of transgenic plants was increased significantly， and monosaccharides that constituted cellulose and hemicellulose showed no significant change.
Populus simonii × P. nigra / drought stress / transcription factor / genetic transformation
S792.11
| 1 | 山仑，邓西平，康绍忠.我国半干旱地区农业用水现状及发展方向［J］.水利学报，2022（9）：27-31. |
| 1 | SHAN L， DENG X P， KANG S Z.Current situation and perspective of agricultural water used in semiarid area of China［J］.Journal of Hydraulic Engineering，2022（9）：27-31. |
| 2 | 许恩银，聂影，芮晓东.基于森林资源清查数据的林地利用效率变化研究［J］.南京林业大学学报（自然科学版），2022，46（5）：213-220. |
| 2 | XU E Y， NIE Y， RUI X D.Analysis on the forest land use efficiency changes based on forest resource inventory data［J］.Journal of Nanjing Forestry University （Natural Sciences Edition），2022，46（5）：213-220. |
| 3 | BANDURSKA H.Drought stress responses：coping strategy and resistance［J］.Plants，2022，11（7）：922. |
| 4 | BRéDA N，HUC R， GRANIER A，et al.Temperate forest trees and stands under severe drought：a review of ecophysiological responses，adaptation processes and long-term consequences［J］.Annals of Forest Science，2006，63（6）：625-644. |
| 5 | FICHOT R， LAURANS F， MONCLUS R，et al.Xylem anatomy correlates with gas exchange，water-use efficiency and growth performance under contrasting water regimes：evidence from Populus deltoides × Populus nigra hybrids［J］.Tree Physiology，2009，29（12）：1537-1549. |
| 6 | LIMOUSIN J M， LONGEPIERRE D，HUC R，et al.Change in hydraulic traits of mediterranean Quercus ilex subjected to long-term throughfall exclusion［J］.Tree Physiology，2010，30（8）：1026-1036. |
| 7 | VENTURAS M D， SPERRY J S， HACKE U G.Plant xylem hydraulics：What we understand，current research，and future challenges［J］.Journal of Integrative Plant Biology，2017，59（6）：356-389. |
| 8 | TONG S F， WANG Y B， CHEN N N，et al.Ptonf-yc9-srmt-ptord26 module regulates the high saline tolerance of a triploid poplar［J］.Genome Biology，2022，23（1）：148. |
| 9 | LI S， LIN Y C J， WANG P Y. et al.The AREB1 transcription factor influences histone acetylation to regulate drought responses and tolerance in Populus trichocarpa ［J］.The Plant Cell，2019，31（3）：663-686. |
| 10 | 杜兆伟，郑唐春，李爽，等.小黑杨快繁与再生体系的优化［J］.植物研究，2015，35（6）：904-907. |
| 10 | DU Z W， ZHENG T C， LI S，et al.Rapid propagation and regeneration system of Populus simonii×Populus nigra ［J］.Bulletin of Botanical Research，2015，35（6）：904-907. |
| 11 | 李晶，王福森，李树森，等.几个杨树新品系抗寒性测定试验［J］.防护林科技，2012（1）：59-61. |
| 11 | LI J， WANG F S， LI S S，et al.Cold-resistance determination for several new strains of poplar［J］.Protection Forest Science and Technology，2012（1）：59-61. |
| 12 | 何旭，高源，张群野，等.白城小黑杨遗传转化体系建立及其应用［J］.植物研究，2023，43（5）：667-678. |
| 12 | HE X， GAO Y， ZHANG Q Y，et al.Establishment and application of genetic transformation system for Populus simonii×P.nigra ‘Baicheng’［J］.Bulletin of Botanical Research，2023，43（5）：667-678. |
| 13 | ILYAS M， NISAR M， KHAN N，et al.Drought tolerance strategies in plants：a mechanistic approach［J］.Journal of Plant Growth Regulation，2020，40（3）：926-944. |
| 14 | PASCUAL M B， DE LA TORRE F， CA?AS R A，et al.NAC transcription factors in woody plants［J］.Progress in Botany，2018，80：195-222. |
| 15 | HAN K J， ZHAO Y， SUN Y H，et al.NACs，generalist in plant life［J］.Plant Biotechnology Journal，2023，21（12）：2433-2457. |
| 16 | NURUZZAMAN M， MANIMEKALAI R， SHARONI A M，et al.Genome-wide analysis of NAC transcription factor family in rice［J］.Gene，2010，465（1/2）：30-44. |
| 17 | JEONG J S， KIM Y S， BAEK K H，et al.Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions［J］.Plant Physiology，2010，153（1）：185-197. |
| 18 | CHEN D D， CHAI S C， MCINTYRE C L，et al.Overexpression of a predominantly root-expressed NAC transcription factor in wheat roots enhances root length，biomass and drought tolerance［J］.Plant Cell Reports，2018，37（2）：225-237. |
| 19 | YANG C F， HUANG Y Z， LV W H，et al. GmNAC8 acts as a positive regulator in soybean drought stress［J］.Plant Science，2020，293：110442. |
| 20 | WANG H L， YANG Q， TAN S Y，et al.Regulation of cytokinin biosynthesis using PtRD26pro-IPT module improves drought tolerance through PtARR10-PtYUC4/5-mediated reactive oxygen species removal in Populus ［J］.Journal of Integrative Plant Biology，2022，64（3）：771-786. |
| 21 | CHEN Z H， PENG Z X， LIU S Q，et al.Overexpression of PeNAC122 gene promotes wood formation and tolerance to osmotic stress in poplars［J］.Physiologia Plantarum，2022，174（4）：e13751. |
| 22 | 李开隆，杨传平，刘桂丰.黑龙江省杨树遗传育种研究进展［J］.东北林业大学学报，2003，31（4）：45-48. |
| 22 | LI K L， YANG C P， LIU G F.Research Progresses of genetics and breeding of Populus in Heilongjiang province［J］.Journal of Northeast Forestry University，2003，31（4）：45-48. |
| 23 | 吴丽丽，高福玲，王雷，等.杨树幼茎特异表达基因及PsnLAC基因的克隆［J］.东北林业大学学报，2011，39（4）：5-7. |
| 23 | WU L L， GAO F L， WANG L，et al.Specific gene expression in stem of Populus simonii×P.nigra and cloning of PsnLAC gene［J］.Journal of Northeast Forestry University，2011，39（4）：5-7. |
| 24 | 王遂，刘梦然，黄海娇，等.转TaLEA基因小黑杨抗寒株系的筛选［J］.东北林业大学学报，2011，39（9）：5-7. |
| 24 | WANG S， LIU M R， HUANG H J，et al.Selection of cold resistant strains from TaLEA gene transferred Populus simonii×P.nigra ［J］.Journal of Northeast Forestry University，2011，39（9）：5-7. |
| 25 | WANG S J， FAN Y， DU S H. et al.PtaERF194 inhibits plant growth and enhances drought tolerance in poplar［J］.Tree Physiology，2022，42（8）：1678-1692. |
| 26 | RODRIGUEZ-ZACCARO F D， GROOVER A.Wood and water：How trees modify wood development to cope with drought［J］.Plants，People，Planet，2019，1（4）：346-355. |
| 27 | ECKERT C， SHARMIN S， KOGEL A，et al.What makes the wood？Exploring the molecular mechanisms of xylem acclimation in hardwoods to an ever-changing environment［J］.Forests，2019，10（4）：358. |
| 28 | BARIGAH T S， CHARRIER O， DOURIS M，et al.Water stress-induced xylem hydraulic failure is a causal factor of tree mortality in beech and poplar［J］.Annals of Botany，2013，112（7）：1431-1437. |
| 29 | LAUDER J D.From the cell to the stand：trait-based approaches to understanding forest response to climate cha-nge［M］.Merced：University of California，2020：101-113. |
| 30 | BANG S W， LEE D K， JUNG H，et al.Overexpression of OsTF1L，a rice HD-Zip transcription factor，promotes lignin biosynthesis and stomatal closure that improves drought tolerance［J］.Plant Biotechnology Journal，2019，17（1）：118-131. |
| 31 | MéNARD D， BLASCHEK L， KRIECHBAUM K，et al.Plant biomechanics and resilience to environmental changes are controlled by specific lignin chemistries in each vascular cell type and morphotype［J］.The Plant Cell，2022，34（12）：4877-4896. |
| 32 | SAINI R， KAUR A， SAINI J K，et al.Trends in lignin biotransformations for bio-based products and energy applications［J］.BioEnergy Research，2023，16（1）：88-104. |
| 33 | ZAHRA N， HAFEEZ M B， KAUSAR A，et al.Plant photosynthetic responses under drought stress：effects and management［J］.Journal of Agronomy and Crop Science，2023，209（5）：651-672. |
/
| 〈 |
|
〉 |
AI Summary
