Determination of the mass fraction of arbutin in Que Zui tea by HPLC

NI Qin, WANG Ru-xiong, LI Jun-xuan, LAI Guo-fang

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Journal of Yunnan University of Nationalities(Natural Sciences Edition) ›› 2025, Vol. 34 ›› Issue (03) : 277-283. DOI: 10.3969/j.issn.1672-8513.2025.03.004

Determination of the mass fraction of arbutin in Que Zui tea by HPLC

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Abstract

A chromatographic column (250 mm × 4.6 mm, 5 μm) with octadecylsilane - bonded silica gel as the filler was used, with methanol and a volume fraction of 1% phosphoric acid aqueous solution as the mobile phase, to establish a method for determining the mass fraction of arbutin in tea using high - performance liquid chromatography (HPLC). The method demonstrates a good linear relationship within the mass concentration range of 0.349 to 402.788 μg/mL, with satisfactory precision, stability, and repeatability. Furthermore, the extraction method for arbutin in tea was optimized with respect to extraction procedures and solvents. This method not only provides a reliable analytical technique for determining the mass fraction of arbutin in tea but also offers new insights for detecting active ingredients in tea and other plant - based products.

Key words

Que Zui tea / arbutin / extraction method optimization / mass fraction determination

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NI Qin , WANG Ru-xiong , LI Jun-xuan , et al. Determination of the mass fraction of arbutin in Que Zui tea by HPLC. Journal of Yunnan University of Nationalities(Natural Sciences Edition). 2025, 34(03): 277-283 https://doi.org/10.3969/j.issn.1672-8513.2025.03.004

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