HPLC法测定雀嘴茶中熊果苷的质量分数

倪芹, 王汝雄, 李俊璇, 来国防

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PDF(955 KB)
云南民族大学学报(自然科学版) ›› 2025, Vol. 34 ›› Issue (03) : 277-283. DOI: 10.3969/j.issn.1672-8513.2025.03.004
农业与食品

HPLC法测定雀嘴茶中熊果苷的质量分数

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Determination of the mass fraction of arbutin in Que Zui tea by HPLC

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摘要

采用十八烷基硅烷键合硅胶为填充剂的色谱柱(250 mm × 4.6 mm,5 μm),以甲醇和体积分数为0.1%的磷酸水溶液为流动相, 建立了高效液相色谱(HPLC)测定雀嘴茶中熊果苷质量分数的方法,该方法在0.349 ~ 402.788 μg/mL质量浓度范围内具有良好的线性关系,精密度、稳定性、重复性均较好,并优化了提取雀嘴茶中熊果苷的方法.这不仅为雀嘴茶中熊果苷的质量分数测定提供了可靠的分析手段,也为茶叶及其他植物性产品中的活性成分检测提供了新思路.

Abstract

A chromatographic column (250 mm × 4.6 mm, 5 μm) with octadecylsilane - bonded silica gel as the filler was used, with methanol and a volume fraction of 1% phosphoric acid aqueous solution as the mobile phase, to establish a method for determining the mass fraction of arbutin in tea using high - performance liquid chromatography (HPLC). The method demonstrates a good linear relationship within the mass concentration range of 0.349 to 402.788 μg/mL, with satisfactory precision, stability, and repeatability. Furthermore, the extraction method for arbutin in tea was optimized with respect to extraction procedures and solvents. This method not only provides a reliable analytical technique for determining the mass fraction of arbutin in tea but also offers new insights for detecting active ingredients in tea and other plant - based products.

关键词

雀嘴茶 / 熊果苷 / 提取方法优化 / 质量分数测定

Key words

Que Zui tea / arbutin / extraction method optimization / mass fraction determination

中图分类号

R286.2

引用本文

导出引用
倪芹 , 王汝雄 , 李俊璇 , . HPLC法测定雀嘴茶中熊果苷的质量分数. 云南民族大学学报(自然科学版). 2025, 34(03): 277-283 https://doi.org/10.3969/j.issn.1672-8513.2025.03.004
NI Qin, WANG Ru-xiong, LI Jun-xuan, et al. Determination of the mass fraction of arbutin in Que Zui tea by HPLC[J]. Journal of Yunnan University of Nationalities(Natural Sciences Edition). 2025, 34(03): 277-283 https://doi.org/10.3969/j.issn.1672-8513.2025.03.004

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基金

云南省“生物医药领域”科技计划项目(2019ZF010)

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