PDF(955 KB)
HPLC法测定雀嘴茶中熊果苷的质量分数
倪芹, 王汝雄, 李俊璇, 来国防
PDF(955 KB)
PDF(955 KB)
HPLC法测定雀嘴茶中熊果苷的质量分数
Determination of the mass fraction of arbutin in Que Zui tea by HPLC
采用十八烷基硅烷键合硅胶为填充剂的色谱柱(250 mm × 4.6 mm,5 μm),以甲醇和体积分数为0.1%的磷酸水溶液为流动相, 建立了高效液相色谱(HPLC)测定雀嘴茶中熊果苷质量分数的方法,该方法在0.349 ~ 402.788 μg/mL质量浓度范围内具有良好的线性关系,精密度、稳定性、重复性均较好,并优化了提取雀嘴茶中熊果苷的方法.这不仅为雀嘴茶中熊果苷的质量分数测定提供了可靠的分析手段,也为茶叶及其他植物性产品中的活性成分检测提供了新思路.
A chromatographic column (250 mm × 4.6 mm, 5 μm) with octadecylsilane - bonded silica gel as the filler was used, with methanol and a volume fraction of 1% phosphoric acid aqueous solution as the mobile phase, to establish a method for determining the mass fraction of arbutin in tea using high - performance liquid chromatography (HPLC). The method demonstrates a good linear relationship within the mass concentration range of 0.349 to 402.788 μg/mL, with satisfactory precision, stability, and repeatability. Furthermore, the extraction method for arbutin in tea was optimized with respect to extraction procedures and solvents. This method not only provides a reliable analytical technique for determining the mass fraction of arbutin in tea but also offers new insights for detecting active ingredients in tea and other plant - based products.
Que Zui tea / arbutin / extraction method optimization / mass fraction determination
R286.2
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