串联索氏提取法快速测定烟草种子中油脂质量分数

王华顺, 张玲芳, 刘华银, 王晋, 顾学娇, 郑朝胚, 刘铭心, 李银科

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云南民族大学学报(自然科学版) ›› 2025, Vol. 34 ›› Issue (01) : 46-50. DOI: 10.3969/j.issn.1672-8513.2025.01.006
农业与食品

串联索氏提取法快速测定烟草种子中油脂质量分数

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Rapid determination of oil content in tobacco seeds by serial Soxhlet extraction

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摘要

传统索氏提取法测定油脂的质量分数周期长、有机溶剂消耗大.为此,选取7个典型的烟草种子样品,包括烤烟、晾晒烟、白肋烟和香料烟,建立了基于串联石油醚索氏提取的快速测定方法.该方法通过将多种样品分别置于不同重叠串联的样品筒内,利用1套索氏提取器同时提取10种以上样品.随后,通过提取前后的样品质量差计算油脂的质量分数.该方法的建立为烟草种子中油脂质量分数的测定提供了快速、准确的新分析途径.对于火龙果籽、芝麻、油菜籽和紫苏籽等油料同样适用,表明该方法具备良好的普遍适应性.

Abstract

Traditional Soxhlet extraction for measuring oil content is time-consuming and consumes a large amount of organic solvents. To solve these problems, seven typical tobacco seed samples, including flue-cured tobacco, sun-cured tobacco, burley tobacco and aromatic tobacco were selected to establish a rapid determination method based on serial petroleum ether Soxhlet extraction. This method extracts more than ten samples by placing multiple samples in different overlapping and tandem sample tubes using a single Soxhlet extractor. Subsequently, the oil content is calculated by the difference in the samples before and after extraction. The establishment of this method provides a new, rapid and accurate analytical approach for measuring oil content in tobacco seeds. This method is also applicable to oilseeds such as dragon fruit seeds, sesame seeds, rapeseeds, and perilla seeds, which indicates its good general applicability.

关键词

串联索氏提取法 / 油脂 / 烟草 / 种子

Key words

serial Soxhlet extraction / oil / tobacco / seeds

中图分类号

O652.62

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导出引用
王华顺 , 张玲芳 , 刘华银 , . 串联索氏提取法快速测定烟草种子中油脂质量分数. 云南民族大学学报(自然科学版). 2025, 34(01): 46-50 https://doi.org/10.3969/j.issn.1672-8513.2025.01.006
WANG Hua-shun, ZHANG Ling-fang, LIU Hua-yin, et al. Rapid determination of oil content in tobacco seeds by serial Soxhlet extraction[J]. Journal of Yunnan University of Nationalities(Natural Sciences Edition). 2025, 34(01): 46-50 https://doi.org/10.3969/j.issn.1672-8513.2025.01.006

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基金

云南中烟工业有限责任公司科研项目(2022JY02)

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