During the wildlife diversity survey in Nanshan National Park, Hunan Province, two amphibian samples (one female and one male) were collected from Baiyun Lake (26°18′36.23″N, 110°20′05.43″E, 606 m above sea level) on April 21, 2023. The morphological traits of the samples suggested that they were related to Nidirana guibeiensis. After PCR amplification and sequencing of mitochondrial 16S rRNA gene fragments from the collected samples, a Bayesian phylogenetic tree of some Nidirana species was constructed. The results showed that the samples were clustered into one branch with N. guibeiensis and had a high support rate (1.00). The genetic distances between the collected Nidirana samples and those from Xing’an County, Guang-xi Province, as determined by the Kimura two-parameter model, were 0, much less than the genetic dis-tances among Nidirana species (3.2%~8.7%). Based on these analyses, the samples were identified as N. gui-beiensis, a new record species of amphibian in Hunan Province.