The spatiotemporal specific expression of genes plays an important regulatory role in organism development and disease occurrence. N6-Methyladenosine (m6A) is the most common epigenetic modification in eukaryotic RNAs. The m6A modification ensures timely and accurate gene expression by changing RNA structure and the interaction between RNAs and RNA-binding proteins, and regulating RNA splicing, sub-cellular localization, translation and stability. Recent studies have shown that m6A not only plays an impor-tant role in the development of organisms, but also is involved in the occurrence of various diseases by changing cell functions. This review focused on the function and mechanism of m6A modification in mam-malian physiological development and disease occurrence, hoping to provide a theoretical basis for using m6A modification in clinical treatment.