Accurate species identification is a prerequisite for biodiversity conservation and management. Mangshan Mountain, located in the south of Hunan and at the border of Hunan and Guangdong provinces, is a priority area for biodiversity protection in Nanling Mountains, with rich diversity of amphibian species. Ac-cording to the Fauna Hunan: Amphibia, there are two Boulenophrys species distributed in Mangshan: B. kua-tunensis and B. brachykolos, but with the further study of the systematic classification of subfamily Mego-phryinae, the distribution of the two species in Mangshan is currently controversial. During May to June, July to August, November 2021 and June to August 2022, four field surveys on the species of Boulenophrys in Mangshan National Nature Reserve, Hunan Province were conducted, and 20 specimens were collected. Through morphological and molecular identification, these specimens proved to belong to four species: B. nanlingensis, B. cf. ombrophila, B. jiulianensis, and B. shimentaina, and the latter three species are new records in Hunan Province. However, the species B. kuatunensis and B. brachykolos were not found to exist in Mangshan. Therefore, the existence of the two species in Mangshan should be further confirmed. In addi-tion, the morphological characteristics of female B. shimentaina and B. nanlingensis were described for the first time, which were lack in the original new record description.