The Yunnan-Guizhou Plateau is one of the regions with most diverse and unique wild species of Gesneriaceae plants in China, and more and more new taxa of the family have been discovered and reported in recent years. Herein, eight newly recorded Gesneriaceae species from Guizhou Province are reported, which include Petrocosmea forrestii Craib, Petrocosmea oblata Craib, Petrocosmea sinensis Oliv., Oreocharis stewardii (Chun) Mich. M?觟ller & A. Weber, Petrocodon chongqingensis F. Wen, B. Pan & L. Y. Su, Paraboea angustifolia Yan Liu & W. B. Xu, Hemiboea pseudomagnibracteata B. Pan & W. H. Wu, Lysionotus feng-shanensis Yan Liu & D. X. Nong. The voucher specimens of the species are preserved in the Tree Herbarium of the School of Forestry, Guizhou University (GZAC).