A preliminary investigation of the species, distribution and floristic characteristics of Impatiens in Guizhou Province has been carried out for more than ten years through field investigation, relevant literature review and specimen research. The results obtained are as follows: 1) There are 69 species (including one variety) of Impatiens L. in Guizhou. They are obviously distributed in narrow range with endemism, and grow more in the south of Guizhou than the north; 2) In terms of geographical composition, most of the Impatiens plants in Guizhou Province are region-specific and belong to tropical Asia and temperate Asia distribution types. Among them, 62 species are endemic to China (89.86%), 10 endemic to Southwest China (14.49%) and 10 endemic to Guizhou (14.49%); 3) Due to their primitive and evolutionary taxa and the characteristics of transition from tropical to temperate zones, the Impatiens plants in Guizhou are probably parts of the evolu-tionary center of Balsaminaceae in China. This investigation is beneficial to understanding the species, geo-graphical distribution pattern and floristic components and characteristics of Impatiens in Guizhou. It also provides reference for studying plant diversity in this province.