2023年4月21日, 在湖南南山国家公园开展野生动物多样性调查过程中, 于白云湖(26°18′36.23″N, 110°20′05.43″E; 海拔606 m)采集到2号蛙类标本(1雌1雄), 经初步形态鉴定, 其与桂北琴蛙(Nidirana guibeiensis)特征相似。对采集标本的线粒体16S rRNA进行PCR扩增测序后, 构建琴蛙属(Nidirana)部分物种贝叶斯系统发育树, 结果显示该蛙类标本与桂北琴蛙聚为一支, 且具有较高的支持率(1.00); 基于Kimura双参数模型估算, 该标本与广西兴安县采集的桂北琴蛙标本间的遗传距离为0, 远小于琴蛙属不同种间的遗传距离(3.2%~8.7%)。经形态特征鉴定和系统发育分析, 确定该蛙类标本为无尾目(Anura)蛙科(Ranidae)琴蛙属(Nidirana)桂北琴蛙, 为湖南省两栖动物新记录种。

During the wildlife diversity survey in Nanshan National Park, Hunan Province, two amphibian samples (one female and one male) were collected from Baiyun Lake (26°18′36.23″N, 110°20′05.43″E, 606 m above sea level) on April 21, 2023. The morphological traits of the samples suggested that they were related to Nidirana guibeiensis. After PCR amplification and sequencing of mitochondrial 16S rRNA gene fragments from the collected samples, a Bayesian phylogenetic tree of some Nidirana species was constructed. The results showed that the samples were clustered into one branch with N. guibeiensis and had a high support rate (1.00). The genetic distances between the collected Nidirana samples and those from Xing’an County, Guang-xi Province, as determined by the Kimura two-parameter model, were 0, much less than the genetic dis-tances among Nidirana species (3.2%~8.7%). Based on these analyses, the samples were identified as N. gui-beiensis, a new record species of amphibian in Hunan Province.