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Comparison of drug resistance of MRSA clinical strains from respiratory tract and non-respiratory tract specimens and their molecular epidemiological characteristics
ERDEMUTU,Yanyan WANG,Yutong LI,Guilin CHEN,Junrui WANG
PDF(626 KB)
PDF(626 KB)
Comparison of drug resistance of MRSA clinical strains from respiratory tract and non-respiratory tract specimens and their molecular epidemiological characteristics
),Junrui WANG3()Objective To discuss the differences in drug resistance， molecular typing， and biofilm-forming abilities of methicillin-resistant Staphylococcus aureus （MRSA） isolated from respiratory and non-respiratory tract specimens. Methods A total of 100 MRSA strains were selected from clinical specimens of the hospitalized patients， and 50 MRSA strains were isolated from the respiratory specimens and another 50 MRSA strains were isolated from non-respiratory tract specimens. These 100 MRSA strains underwent an in vitro susceptibility test for 14 kinds of antimicrobial drugs， molecular typing was performed through multilocus sequence typing （MLST） and Staphylococcus aureus protein A （Spa） typing， and their biofilm-forming abilities were detected by crystal violet staining assay. Results The in vitro susceptibility tests results showed that all the 100 MRSA strains had high overall resistance rates of over 60.0% to penicillin， erythromycin， clindamycin， levofloxacin， ciprofloxacin， moxifloxacin， and tetracycline， while only 9.0% exhibited resistance to trimethoprim-sulfamethoxazole， and all the strains were susceptible to vancomycin， linezolid， tigecycline， and quinupristin/dalfopristin. Compared with those isolated from non-respiratory tract specimens，the MRSA strains isolated from respiratory tract specimens showed significantly higher resistance rates to moxifloxacin， levofloxacin， ciprofloxacin， tetracycline， and gentamicin （P<0.05）. Among the 100 MRSA strains， 17 genotypes were identified， and the dominant types weve ST5-t2460 （26.0%）， ST239-t030 （23.0%）， and ST59-t437 （20.0%）. In the MRSA from the respiratory tract specimens， the dominant types were ST5-t2460 （20.0%） and ST239-t030 （13.0%）， followed by ST59-t437 （7.0%）； in the non-respiratory tract specimens， 13 genotypes were found， and the dominant types were ST59-t437 （13.0%） and ST239-t030 （10.0%）. All 100 MRSA strains were biofilm producers， among them the strong biofilm formers， moderate biofilm formers， and weak biofilm formers accounted for 2.0% （2/100）， 24.0% （24/100）， and 74.0% （74/100）， respectively. The ST59-t437 clones had overall strong biofilm-forming abilities， and 60.0% （12/20） stains were moderate to strong biofilm formers. Conclusion The MRSA strains isolated from the respiratory tract specimens have a significantly higher overall resistance rate than those isolated from the non-respiratory tract specimens. The ST59-t437 and ST239-t030 genotypes are dominant clones common to both types of specimens， and ST59-t437 strains show strong biofilm-forming abilities， and ST239-t030 strains exhibit the strongest resistance.
Methicillin-resistant Staphylococcus aureus / Drug resistance / Molecular typing / Biofilm
R378.1
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