Ameliorative effects of tea on metabolic disorders in obesity mice induced by high-fat diet

WANG Chen, BAN Xiang, LIU Jia-xing, SANG Si-yao, AO Xue, SU Ming-jie, HU Bin-wei, LI Hui

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Fudan University Journal of Medical Sciences ›› 2025, Vol. 52 ›› Issue (03) : 393-402. DOI: 10.3969/j.issn.1672-8467.2025.03.009
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Ameliorative effects of tea on metabolic disorders in obesity mice induced by high-fat diet

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Abstract

Objective To investigate the ameliorative effects and mechanisms of six types of tea （green tea， cyan tea， red tea， white tea， black tea and yellow tea） on metabolic disorders in obesity mice induced by high-fat diet （HFD）. Methods Four-week-old male C57BL/6J mice were randomly divided into 8 groups with 7 mice per group. An HFD-induced obese mouse model was established， and the mice in control group maintained on standard diet followed by intragastric administration of different teas for 5 weeks. The body weight， liver weight ratio， fasting blood glucose， and lipid profile of the mice were measured to assess glucose and lipid metabolism. Serum inflammatory factors including IL-6， tumor necrosis factor-alpha （TNF-α） and oxidative stress markers ［malondialdehyde （MDA） and superoxide dismutase （SOD） were measured. Additionally， liver histopathology and the expression of key glycolipid metabolism-related genes， adenosine monophosphate-activated protein kinase （AMPK） and carnitine palmitoyltransferase 1 （CPT-1）， were analyzed to explore underlying mechanisms. Results Cyan tea significantly suppressed weight gain， demonstrating superior weight control. White tea markedly reduced fasting blood glucose levels and decreased the area under the curve of oral glucose tolerance test （OGTT） and insulin tolerance test （ITT）， indicating synergistic improvements in glucose metabolism and insulin sensitivity. Yellow tea exhibited exceptional anti-inflammatory and antioxidant effects， reducing hepatic IL-6 and MDA while enhancing SOD activity. Green tea activated the lipid oxidation pathway by upregulating AMPK/CPT-1 expression. All kinds of tea significantly attenuated hepatic lipid droplet accumulation. Conclusion All six types of tea alleviated metabolic disorders by reducing hepatic fat content in obesity mice. However， different types of tea exert their unique effects on improving metabolic disorders through differential mechanisms such as glucose metabolism regulation， lipid oxidation， and anti-inflammatory and antioxidant actions.

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tea / high-fat diet （HFD） / obesity / insulin resistance / inflammatory reaction / oxidative stress / metabolic disorders / mouse

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WANG Chen , BAN Xiang , LIU Jia-xing , et al . Ameliorative effects of tea on metabolic disorders in obesity mice induced by high-fat diet. Fudan University Journal of Medical Sciences. 2025, 52(03): 393-402 https://doi.org/10.3969/j.issn.1672-8467.2025.03.009

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王晨 数据收集，论文构思、撰写和修订。班翔 数据统计和分析，论文撰写和修订。刘佳星，桑思瑶，敖雪，苏明杰，胡彬蔚 研究设计，数据收集，文献调研和整理。李辉 研究设计和指导，论文修订。

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National Key Research and Development Program of China(2020YFE0201600)

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