Degradation of 1,2,4-trichlorobenzene by an anaerobic enrichment culture mediated by Dehalobacter species

Lianghua LÜ, Wenjing QIAO, Han ZHANG, Shujun YE, Jichun WU, Shui WANG, Jiandong JIANG

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Earth Science Frontiers ›› 2024, Vol. 31 ›› Issue (2) : 472-480. DOI: 10.13745/j.esf.sf.2022.10.41

Degradation of 1,2,4-trichlorobenzene by an anaerobic enrichment culture mediated by Dehalobacter species

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Abstract

1,2,4-Trichlorobenzene (TCB) is a typical organic contaminant in soil and groundwater at industrial plant in China. 1,2,4-TCB is recalcitrant, bio-accumulative and highly toxic, posing serious threats to the environment and human health. 1,2,4-TCB is denser than water and easily penetrates into an anaerobic environment. Therefore, understanding the anaerobic biotransformation of 1,2,4-TCB is of great practical significance to on-site remediation. In this study, an anaerobic enrichment culture, which can sustainably dechlorinate 1,2,4-TCB to MCB via 1,4-DCB, is obtained. 16S rRNA gene amplicon sequencing and qPCR demonstrated Dehalobacter is responsible for the observed reductive dechlorination with a growth yield of (1.68±0.8)×106 copies per μmol released Cl-. A complete 16S rRNA gene sequence was obtained through PCR and a corresponding phylogenetic tree was constructed. This study provides a reference for the enrichment culture technique as well as theoretical guidance for the in-situ anaerobic bioremediation of contaminated sites.

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1,2,4-trichlorobenzene / groundwater contamination / bioremediation / Dehalobacter / reductive dechlorination

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Lianghua LÜ , Wenjing QIAO , Han ZHANG , et al . Degradation of 1,2,4-trichlorobenzene by an anaerobic enrichment culture mediated by Dehalobacter species. Earth Science Frontiers. 2024, 31(2): 472-480 https://doi.org/10.13745/j.esf.sf.2022.10.41

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